Evaluation of vitrification for cryopreservation of teeth
Dissanayake Surangi C, 차충민, 최성호, 이승종, 김진,
소속 상세정보
( Dissanayake Surangi C ) - Yonsei University College of Dentistry Department of Oral Pathology Oral Cancer Research Institute
차충민 ( Che Zhong-Min ) - Yonsei University College of Dentistry Department of Oral Pathology Oral Cancer Research Institute
최성호 ( Choi Seong-Ho ) - Yonsei University College of Dentistry Department of Periodontology
이승종 ( Lee Seung-Jong ) - Yonsei University College of Dentistry Department of Conservative Dentistry
김진 ( Kim Jin ) - Yonsei University College of Dentistry Department of Oral Pathology Oral Cancer Research Institute
KMID : 0363020100400030111
Abstract
Purpose The aim of this study was to investigate whether vitrification in the cryopreservation of periodontal ligament (PDL) cells could be useful for tooth banking.
Methods In step 1, primary cultured human PDL cells were cryopreserved in 100% conventional cryopreservation media and 100% vitrification media (ESF40 media) in different temperatures for 2 weeks. In step 2, a series of modified vitrification formulae named T1 (75% vitrification media + 25% F-media), T2 (50% vitrification media + 50% F-media) and T3 (25% vitrification media + 75% F-media) were used to store PDL cells for 2 weeks and 4 weeks in liquid nitrogen. MTT assay was performed to examine the viability of PDL cells.
Results Maximum cell viability was achieved in cells stored in 100% conventional cryopreservation media at -196℃ (positive control group) in step 1. Compared to the positive control group, viability of the cells stored in 100% vitrification media was very low as 10% in all test conditions. In step 2, as the percentage of vitrification media decreased, the cell viability increased in cells stored for 2 weeks. In 4-week storage of cells in step 2, higher cell viability was observed in the T2 group than the other vitrification formulae while the positive control group had the highest viability. There was no statistically significant difference in the cell viability of 2-week and 4-week stored cells in the T2 group.
Conclusions These observations indicate 100% vitrification media is not successful in PDL cell cryopreservation. Conventional cryopreservation media is currently the most appropriate media type for this purpose while T2 media would be interesting to test for long-term storage of PDL cells.
키워드
Cryopreservation; Periodontal ligament; Tissue banks
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